Molecular Tools in Biotechnology MCQ Questions & Answers | Biotechnology

Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially?

CRISPR-Cas system is associated with

Identify the correct statements.
P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain reaction.
Q. S1 nuclease can cleave the DNA strand of a DNARNA hybrid.
R. T4 polynucleotide kinase is used for labeling 3' end of DNA.
S. Baculovirus (Autographa californica) can be used as an insect expression vector.

W, X and Y are the intermediates in a biochemical pathway as shown below:
$$S \cdot \cdot \cdot \cdot \cdot \cdot \cdot \cdot \to W \to X \to Y \to Z$$
Mutants auxotrophic for Z are found in four different complementation groups, namely Z1, Z2, Z3 and Z4. The growth of these mutants on media supplemented with W, X, Y or Z is shown below (Yes: growth observed: No: growth not observed):

Mutants	Media Supplemented with Mutants
	W	X	Y	Z
Z1	No	No	Yes	Yes
Z2	No	Yes	Yes	Yes
Z3	No	No	No	Yes
Z4	Yes	Yes	Yes	Yes

What is the order of the four complementation groups in terms of the step they block?

For amplification of GC rich sequences by polymerase chain reaction, identify the reagent that hinds and stabilizes AT sequences and destabilizes GC regions.

In order to identify the person who committed a crime, forensic experts will need to extract DNA from the tissue sample collected at the crime scene, and conduct one of the following procedures for DNA fingerprinting analysis

Restriction endonucleases from two different organisms that recognize the same DNA sequence for cleavage are called

Protein-DNA interactions in vivo can be studied by

The DNA sequence shown below is to be amplified by PCR:
5' GCTAAGATCTGAATTTTCC . . . . . . . .
TTGGGCAATAATGTAGCGC 3'
3' CGATTCTAGACTTAAAAGG . . . . . . . .
AACCCGTTATTACATCGCG 5'
Which one of the following pair of primers can be used for this amplification?

For site-directed mutagenesis, which one of the following restriction enzymes is used to digest methylated DNA?