Molecular Tools in Biotechnology MCQ Questions & Answers

21.
For the sequence of dsDNA given below, identify the set of primers required to amplify this DNA by PCR.
3' GACTCCA . . . . . . . . TACAACC 5'
5' CTGAGGT . . . . . . . . ATGTTGG 3'

A. 5' GGTTGTA and 5' GACTCCA

B. 5' CTGAGGT and 5' CCAACAT

C. 5' ACTCAGT and 5' ATGTTGG

D. None of the above

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22.
Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).
5'-GACCTGTGG . . . . . . . . ATACGGGAT-3'
3'-CTGGACACC . . . . . . . . TATGCCCTA-5'
Primers
P. 5'-GACCTGTGG-3'
Q. 5'-CCACAGGTC-3'
R. 5'-TAGGGCATA-3'
S. 5'-ATCCCGTAT-3'

A. P and R

B. P and S

C. Q and R

D. Q and S

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23.
Protein binding regions of DNA are identified by one of the following techniques.

A. Finger printing

B. Foot printing

C. Southern blotting

D. Western blotting

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24.
Nick translation of DNA is a method for making DNA probes. Identify from below what is not required for nick translation method.

A. DNA polymerase

B. DNAase

C. Primers

D. Deoxyribonucleotides

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25.
Match the reagents in Group I with their preferred cleavage sites in Group II.

Group I Group II

P. Cyanogen bromide 1. Carboxyl side of methionine

Q. o-Iodosobenzoate 2. Amino side of methionine

R. Hydroxylamine 3. Carboxyl side of tryptophan

S. 2-Nitro-5-thiocyanobenzoate 4. Amino side of cysteine

5. Asparagine-glycine bonds

A. P-1, Q-3, R-5, S-4

B. P-2, Q-3, R-1, S-4

C. P-1, Q-2, R-5, S-4

D. P-4, Q-2, R-5, S-3

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26.
The mobility of DNA in agarose gel electrophoresis is solely based on its

A. Change

B. Conformation

C. Size

D. None of the above

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27.
A polymerase chain reaction was performed beginning with 400 template DNA molecules in a 100 μL reaction. After 20 cycles of polymerase chain reaction, how many molecules of the amplified product will be present in 0.1 μL of reaction?

A. 2.19 × 10⁴

B. 4.19 × 10⁴

C. 2.19 × 10⁵

D. 4.19 × 10⁵

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28.
A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Of the following techniques, which one would verify the gene duplication?

A. Northern blotting

B. Southern blotting

C. South western blotting

D. Western blotting

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29.
All of the following are true about DNA microarray technology except

A. An electron microscope is used to gather data from the arrays

B. The technology is used to assess transcription from multiple genes simultaneously

C. The technology works best for organisms whose genome is completely sequenced

D. The technology is derived from computer chip manufacture

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30.
Expressed Sequence Tag is defined as

A. A partial sequence of a codon randomly selected from cDNA library

B. The characteristic gene expressed in the cell

C. The protein coding DNA sequence of a gene

D. Uncharacterized fragment of DNA presence in the cell

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Group I	Group II
P. Cyanogen bromide	1. Carboxyl side of methionine
Q. o-Iodosobenzoate	2. Amino side of methionine
R. Hydroxylamine	3. Carboxyl side of tryptophan
S. 2-Nitro-5-thiocyanobenzoate	4. Amino side of cysteine
	5. Asparagine-glycine bonds

Molecular Tools in Biotechnology MCQ Questions & Answers | Biotechnology

21. For the sequence of dsDNA given below, identify the set of primers required to amplify this DNA by PCR. 3' GACTCCA . . . . . . . . TACAACC 5' 5' CTGAGGT . . . . . . . . ATGTTGG 3'

Answer & Solution

22. Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR). 5'-GACCTGTGG . . . . . . . . ATACGGGAT-3' 3'-CTGGACACC . . . . . . . . TATGCCCTA-5' Primers P. 5'-GACCTGTGG-3' Q. 5'-CCACAGGTC-3' R. 5'-TAGGGCATA-3' S. 5'-ATCCCGTAT-3'

Answer & Solution

23. Protein binding regions of DNA are identified by one of the following techniques.

Answer & Solution

24. Nick translation of DNA is a method for making DNA probes. Identify from below what is not required for nick translation method.

Answer & Solution

Answer & Solution

26. The mobility of DNA in agarose gel electrophoresis is solely based on its

Answer & Solution

27. A polymerase chain reaction was performed beginning with 400 template DNA molecules in a 100 μL reaction. After 20 cycles of polymerase chain reaction, how many molecules of the amplified product will be present in 0.1 μL of reaction?

Answer & Solution

28. A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Of the following techniques, which one would verify the gene duplication?

Answer & Solution

29. All of the following are true about DNA microarray technology except

Answer & Solution

30. Expressed Sequence Tag is defined as

Answer & Solution

21.
For the sequence of dsDNA given below, identify the set of primers required to amplify this DNA by PCR.
3' GACTCCA . . . . . . . . TACAACC 5'
5' CTGAGGT . . . . . . . . ATGTTGG 3'

22.
Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).
5'-GACCTGTGG . . . . . . . . ATACGGGAT-3'
3'-CTGGACACC . . . . . . . . TATGCCCTA-5'
Primers
P. 5'-GACCTGTGG-3'
Q. 5'-CCACAGGTC-3'
R. 5'-TAGGGCATA-3'
S. 5'-ATCCCGTAT-3'

23.
Protein binding regions of DNA are identified by one of the following techniques.

24.
Nick translation of DNA is a method for making DNA probes. Identify from below what is not required for nick translation method.

26.
The mobility of DNA in agarose gel electrophoresis is solely based on its

27.
A polymerase chain reaction was performed beginning with 400 template DNA molecules in a 100 μL reaction. After 20 cycles of polymerase chain reaction, how many molecules of the amplified product will be present in 0.1 μL of reaction?

28.
A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Of the following techniques, which one would verify the gene duplication?

29.
All of the following are true about DNA microarray technology except

30.
Expressed Sequence Tag is defined as