Recombinant DNA Technology MCQ Questions & Answers | Biotechnology
Learn and Practice Recombinant DNA Technology MCQ Questions & Answers Available for Biotechnology Engineering Students to Clear GATE Exams, Technical Interviews, Competitive Examinations, and Other Entrance Exams. The Recombinant DNA Technology Chapter is Especially Important for Biotechnology Engineering and GATE Students. Page-2 section-1
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Phage T7 promoter containing plasmids are used for over-expression of cloned genes because
Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because
The restriction endonuclease Eco52I recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco52I?
Expression in poor amount and in inactive form of cDNA of a eukaryotic protein in Escherichia coli using its expression vector is due to
P. The absence of capping mechanism of mRNA.
Q. Codon bias.
R. Absence of polyadenylation.
S. Absence of proper glycosylation.