Recombinant DNA Technology MCQ Questions & Answers

51.
Which of the following processes require energy?

A. Ligation

B. Transformation

C. Restriction digestion

D. Hybridization

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52.
A plasmid cloning vector should contain all of the following sequences except

A. Origin of replication

B. Inducible promoter

C. Selectable marker gene

D. Multiple cloning sites

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53.
"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"

A. EcoRI-2; BamHI-1; HindIII-2

B. EcoRI-1; BamHI-1; HindIII-2

C. EcoRI-3; BamHI-2; HindIII-1

D. EcoRI-2; BamHI-2; HindIII-1

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54.
Consider the following statements.
I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase.
II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.

A. Only I is true

B. Both I and II are true

C. Only II is true

D. I is true and II is false

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55.
You have cut the genome of a double-stranded viral genome with a restriction endonuclease and electrophoresed the products on an agarose gel. You observe only one band on the gel, equivalent to the size of the genome. This is because

A. There are no introns in the genome

B. The introns contain the recognition sites and have already been spliced out

C. All of restriction fragments are too small to detect

D. Restriction endonucleases do not cut RNA, and this virus has an RNA genome

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56.
Which one of the following features is not required in a prokaryotic expression vector?

A. oriC

B. Selection marker

C. CMV promoter

D. Ribosome binding site

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57.
Yeast artificial chromosomes (YAC's) are used for cloning

A. Large segments of DNA

B. mRNA

C. Bacterial DNA

D. Yeast DNA

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58.
In baculovirus expression vector, foreign genes are expressed from the promoter of

A. Polyhedrin gene

B. Bacteriophage T7 gene

C. E. coli lacZ gene

D. Yeast phosphoglycerate kinase gene

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Recombinant DNA Technology MCQ Questions & Answers | Biotechnology

51. Which of the following processes require energy?

Answer & Solution

52. A plasmid cloning vector should contain all of the following sequences except

Answer & Solution

Answer & Solution

54. Consider the following statements. I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase. II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.

Answer & Solution

55. You have cut the genome of a double-stranded viral genome with a restriction endonuclease and electrophoresed the products on an agarose gel. You observe only one band on the gel, equivalent to the size of the genome. This is because

Answer & Solution

56. Which one of the following features is not required in a prokaryotic expression vector?

Answer & Solution

57. Yeast artificial chromosomes (YAC's) are used for cloning

Answer & Solution

58. In baculovirus expression vector, foreign genes are expressed from the promoter of

Answer & Solution

51.
Which of the following processes require energy?

52.
A plasmid cloning vector should contain all of the following sequences except

54.
Consider the following statements.
I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase.
II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.

55.
You have cut the genome of a double-stranded viral genome with a restriction endonuclease and electrophoresed the products on an agarose gel. You observe only one band on the gel, equivalent to the size of the genome. This is because

56.
Which one of the following features is not required in a prokaryotic expression vector?

57.
Yeast artificial chromosomes (YAC's) are used for cloning

58.
In baculovirus expression vector, foreign genes are expressed from the promoter of