31.
A gene cannot be isolated from a human genomic DNA library by functional complementation in E. coli because of

32.
During a positive-negative selection process, transformed animal cells expressing . . . . . . . . are killed in presence of ganciclovir in the medium.

36.
Select the correct combination of genetic components that are essential for the transfer of T-DNA segment from Agrobacterium tumefaciens to plant cells.

37.
A linear DNA fragment is 100% labeled at one end and has 3 restriction sites for EcoRI. If it is partially digested by EcoRI so that all possible fragments are produced, how many of these fragments will be labeled and how many will not be labeled?

38.
A cell has five molecules of a rare mRNA. Each cell contains 4 × 105 mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?

40.
The restriction endonuclease HaeIII recognizes the sequence GGCC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice