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Recombinant DNA Technology MCQ Questions & Answers | Biotechnology

Learn and Practice Recombinant DNA Technology MCQ Questions & Answers Available for Biotechnology Engineering Students to Clear GATE Exams, Technical Interviews, Competitive Examinations, and Other Entrance Exams. The Recombinant DNA Technology Chapter is Especially Important for Biotechnology Engineering and GATE Students. Page-4 section-1

31
A gene cannot be isolated from a human genomic DNA library by functional complementation in E. coli because of
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Answer & Solution
Answer: Option B
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32
During a positive-negative selection process, transformed animal cells expressing . . . . . . . . are killed in presence of ganciclovir in the medium.
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Answer: Option B
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33
To isolate a gene coding for glucagon, the cDNA library as to be constructed using mRNA isolated from
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Answer: Option B
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34
Which one of the following cannot be a recognition sequence for a type II restriction enzyme?
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Answer: Option D
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35
To be a cloning vector, a plasmid does not require
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Answer: Option B
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36
Select the correct combination of genetic components that are essential for the transfer of T-DNA segment from Agrobacterium tumefaciens to plant cells.
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Answer: Option B
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37
A linear DNA fragment is 100% labeled at one end and has 3 restriction sites for EcoRI. If it is partially digested by EcoRI so that all possible fragments are produced, how many of these fragments will be labeled and how many will not be labeled?
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Answer: Option A
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38
A cell has five molecules of a rare mRNA. Each cell contains 4 × 105 mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?
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Answer: Option D
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39
The enzymes that can be used in 5' end labeling of DNA are
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Answer: Option A
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40
The restriction endonuclease HaeIII recognizes the sequence GG↓CC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice
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Answer: Option C
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