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Recombinant DNA Technology MCQ Questions & Answers | Biotechnology

Learn and Practice Recombinant DNA Technology MCQ Questions & Answers Available for Biotechnology Engineering Students to Clear GATE Exams, Technical Interviews, Competitive Examinations, and Other Entrance Exams. The Recombinant DNA Technology Chapter is Especially Important for Biotechnology Engineering and GATE Students. Page-3 section-1

21
Restriction endonucleases which recognize and cut same recognition sequences are known as
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Answer & Solution
Answer: Option A
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22
The length of each boarder sequence in Ti plasmid is about
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Answer: Option D
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23
A DNA fragment of 4500 bp has to be tailed with dT residues by using dTTP and the enzyme 'terminal transferase' The stock solution of dTTP that is used as a substrate has concentration of 150 μM. Ten μL of this stock solution is added to a total volume of 200 μL reaction. What will be the concentration of dTTP in the reaction?
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Answer: Option A
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24
Baculovirus expression system is used to express heterologous genes in
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Answer: Option C
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25
A variety of genetic elements are used in the transgenic plant research. Match the genetic elements (Group I) with their corresponding source (Group II).
Group I Group II
P. Ubiquitin1 promoter 1. Agrobacterium tumefaciens
Q. Nos transcriptional terminator 2. Streptomyces hygroscopicus
R. bar selection marker gene 3. Escherichia coli
S. gus reporter gene 4. Zea mays
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Answer: Option D
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26
The essential component of Ti plasmid required for integration into plant genome is
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Answer: Option B
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27
The bacteria known to be naturally competent for transformation of DNA is
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Answer: Option B
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28
BAC, which can be used to clone large DNA fragments, is derived from
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Answer: Option B
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29
Transfer of T-DNA from Ti plasmid into plant cell is mediated by
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Answer: Option B
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30
Antibiotic resistance marker that cannot be used in a cloning vector in Gram negative bacteria is
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Answer: Option C
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