CO₂ fixation during C₄ pathway occurs in the chloroplast of

CO₂ fixation during C₄ pathway occurs in the chloroplast of Bundle sheath cells.
In C₄ cycle of photosynthesis, where a four-carbon compound is produced, unique leaf anatomy allows carbon dioxide to concentrate in 'bundle sheath' cells around Rubisco.
This structure delivers carbon dioxide straight to Rubisco, effectively removing its contact with oxygen and the need for photorespiration.