Cryopreservation can not be done for microorganisms which are

Cryopreservation cannot be done for Aerobic microorganisms.
Cryopreservation is a process where organelles, cells, tissues, extracellular matrix, organs, or any other biological constructs susceptible to damage caused by unregulated chemical kinetics are preserved by cooling to very low temperatures.
Cells might be damaged if the intracellular concentration of solutes is high. The leakage of certain solutes from the cell during freezing should be checked. The physiological status of the plant material is also essential.