Disease free plants in micropropagation can be obtained through

Disease-free plants in micro-propagation can be obtained through meristem culture.
Meristem culture is a tissue culture technique that uses an apical meristem with 1 - 3 leaf primordia to prepare vegetative propagation clones of a plant.
The main aim of this culture is to obtain disease-free plants, and shoot tips of up to 10 mm are used. For rapid clonal propagation, a shoot-tip culture is followed in which (5 - 10 mm) explants are used. Hence, the majority of meristem cultures are essentially shoot-tip cultures.
It happens because its active cell division reduces the differentiation of vascular tissues.