Phytoplasma can be detected by

Phytoplasma can be detected by DAPI stain, Dienes' stain, and ELISA.
Phytoplasmas are obligate intracellular parasites of plant phloem tissue and of the insect vectors that are involved in their plant-to-plant transmission.
The use of the polymerase chain reaction (PCR) and sequencing of the corresponding amplicons generated from the ribosomal RNA (rRNA) gene have allowed the identification and comparison of phytoplasma strains with techniques such as restriction fragment length polymorphism (RFLP) and phylogenetic analysis.