The seed vigour test which determines activities of decarboxylation is

The seed vigour test which determines the activities of decarboxylation is the ATPase test.
Seed vigour is an important quality parameter that needs to be assessed to supplement germination and viability tests to gain insight into the performance of a seed lot in the field or storage.
The ATPase assay measures the ADP produced in the reaction from ATP. The amount of ADP released indicates the robustness of the reaction. More ADP equates to a more active enzyme.