Knott's technique is a concentration method for detection of following parasite in blood

Knott's technique is a concentration method for the detection of Microfilariae parasites in blood.
This technique is used to recover low numbers of microfilariae from blood. A solution is used to lyse the RBCs in a large blood sample, and the organisms are concentrated from the supernatant fluid by centrifugation.
It is easy to perform, rapid and inexpensive, it conserves mf morphology and size, and it enhances the sensitivity of mf detection in blood samples.