Samples suspected for Pox virus are inoculated in embryonated eggs by the route of

Samples suspected of Pox virus are inoculated in embryonated eggs by the route of CAM.
Embryonated chicken eggs are inoculated by the allantoic route at approximately the middle of the 21 days embryonation period, at 8 - 10 days of embryonation; they are inoculated by the amniotic route late in the incubation period, at 14 - 16 days of embryonation.