Analytical Tools in Biotechnology MCQ Questions & Answers

41

Which one of the following techniques can be used to determine the structure of a 15 kDa globular protein at atomic resolution?

A) Raman spectroscopy B) IR spectroscopy C) UV spectroscopy D) NMR spectroscopy

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Answer & Solution

Answer: Option D

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42

Which of the following has to be computed to determine transmittance and absorbance at various frequencies?

A) Ratio of signal and noise B) Ratio of sample and reference spectra C) Sample spectra D) Reference spectra

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Answer & Solution

Answer: Option B

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43

Which of the following is the function of the chopper in Atomic Absorption Spectroscopy?

A) To split the beam into two B) To break the steady light into a pulsating light C) To filter unwanted components D) To reduce the sample into atomic state

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Answer & Solution

Answer: Option B

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44

Which of the following is not a fuel used in flame photometry?

A) Acetylene B) Propane C) Hydrogen D) Camphor oil

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Answer & Solution

Answer: Option D

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45

Determine the correctness or otherwise of the following Assertion (A) and the Reason (R).
Assertion: MTT assay is used to determine cell viability based on the principle of color formation by DNA fragmentation.
Reason: MTT assay is used to determine cell viability based on the color development by converting tetrazolium soluble sail to insoluble salt.

A) Both (A) and (R) are true and (R) is the correct reason for (A) B) Both (A) and (R) are true and (R) is not the correct reason for (A) C) (A) is true but (R) is false D) (A) is false but (R) is true

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Answer & Solution

Answer: Option A

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46

Which of the following is not a limitation of Beer Lambert's law, which gives the relation between absorption, thickness and concentration?

A) Concentration must be lower B) Radiation must have higher bandwidth C) Radiation source must be monochromatic D) Does not consider factors other than thickness and concentration that affect absorbance

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Answer & Solution

Answer: Option B

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47

Phototubes are more sensitive than photovoltaic cells.

A) True B) False

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Answer & Solution

Answer: Option A

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48

Velocity of electromagnetic radiation is more in a vacuum than in any medium.

A) True B) False

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Answer & Solution

Answer: Option A

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49

Which of the following is the wavelength of microwave radiation?

A) 10 - 780nm B) 0.78 - 30µm C) 0.6 - 10 m D) 0.75 - 3.75 mm

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Answer & Solution

Answer: Option D

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50

Which of the following is not a detector used in Flame emission photometers?

A) Photronic cell B) Photovoltaic cell C) Photoemissive tube D) Chromatogram

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Answer & Solution

Answer: Option D

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Analytical Tools in Biotechnology MCQ Questions & Answers | Biotechnology