Basic Principles of Biotechnology MCQ Questions & Answers

1.
Which of the following is a prime example of a ribonucleoprotein enzyme, the catalytic activity of which (under non-physiological conditions), resides solely in its RNA component

A. Reverse transcriptase

B. Cytochrome c

C. Bacterial RNA polymerase

D. RNase P

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2.
Main functions of baffles in a bioreactor are
P. To prevent a vortex.
Q. To increase aeration.
R. To reduce interfacial area of oxygen transfer.
S. To reduce aeration rate.

A. P, Q

B. Q, R

C. R, S

D. P, S

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3.
All the following may be methods for the inhibition of microbial growth by antibiotics except

A. Antibiotics disrupt cell wall synthesis

B. Antibiotics interfere with cell membrane function

C. Antibiotics prevent the release of energy from ATP

D. Antibiotics inhibit the synthesis of protein

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4.
A feature common to almost all transmembrane proteins is

A. A phosphorylated exterior domain

B. A structure consisting almost exclusively of beta-sheets

C. An amino acid sequence rich in acidic residues

D. An alpha-helical region of about 20 to 25 hydrophobic amino acids

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5.
The distribution of transmembrane protein in the palne of a cell membrane can best be visualized by which of the following:

A. Thin-section electron microscopy

B. Freeze-fracture electron microscopy

C. Scanning electron microscopy

D. SDS gel electrophoresis

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6.
Broker, Chow, Roberts and P. Sharp hybridized mRNA from adenovirus to denatured adenovirus DNA and examined the resultant duplex molecules by electron microscopy. Which of the following did they observe?

A. mRNA and DNA formed a continuous duplex throughout the length of the RNA

B. mRNA and DNA formed a continuous duplex throughout the length of DNA

C. On one strand there were looped out regions of single-stranded RNA

D. On one strand there were looped out regions of single-stranded DNA

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7.
If a mutation occurred in the phosphorylation site in the CTD domain of RNA polymerase II, which of the following outcomes would be observed?

A. Transcription of tRNA would occur normally

B. Transcription of mRNA would not occur

C. Transcription of precursor rRNA would not occur

D. All of the above

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8.
A microorganism when viewed under a compound microscope with objective of 40X and eye piece of 10X magnification measured 400μ in length. The same microorganism when viewed under a dissecting microscope with 10X lens, would measure

A. 100μ

B. 10μ

C. 40μ

D. 400μ

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9.
Which of the following technique is used to inactivate a gene by altering the DNA encoding it?

A. Homologous recombination

B. Antisense nucleic acid blocks

C. Antibody microinjection

D. Introduction of dominant inhibitory mutants

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10.
Gene conversion is observed

A. During meiosis, one allele is replaced by another allele

B. During meiosis, the expected 2 : 2 ratio of alleles is replaced with a 4 : 0 ratio

C. During meiosis, the expected 2 : 2 ratio of alleles is replaced with a 3 : 1 ratio

D. All of the above

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Basic Principles of Biotechnology MCQ Questions & Answers | Biotechnology

1. Which of the following is a prime example of a ribonucleoprotein enzyme, the catalytic activity of which (under non-physiological conditions), resides solely in its RNA component

Answer & Solution

2. Main functions of baffles in a bioreactor are P. To prevent a vortex. Q. To increase aeration. R. To reduce interfacial area of oxygen transfer. S. To reduce aeration rate.

Answer & Solution

3. All the following may be methods for the inhibition of microbial growth by antibiotics except

Answer & Solution

4. A feature common to almost all transmembrane proteins is

Answer & Solution

5. The distribution of transmembrane protein in the palne of a cell membrane can best be visualized by which of the following:

Answer & Solution

6. Broker, Chow, Roberts and P. Sharp hybridized mRNA from adenovirus to denatured adenovirus DNA and examined the resultant duplex molecules by electron microscopy. Which of the following did they observe?

Answer & Solution

7. If a mutation occurred in the phosphorylation site in the CTD domain of RNA polymerase II, which of the following outcomes would be observed?

Answer & Solution

8. A microorganism when viewed under a compound microscope with objective of 40X and eye piece of 10X magnification measured 400μ in length. The same microorganism when viewed under a dissecting microscope with 10X lens, would measure

Answer & Solution

9. Which of the following technique is used to inactivate a gene by altering the DNA encoding it?

Answer & Solution

10. Gene conversion is observed

Answer & Solution

1.
Which of the following is a prime example of a ribonucleoprotein enzyme, the catalytic activity of which (under non-physiological conditions), resides solely in its RNA component

2.
Main functions of baffles in a bioreactor are
P. To prevent a vortex.
Q. To increase aeration.
R. To reduce interfacial area of oxygen transfer.
S. To reduce aeration rate.

3.
All the following may be methods for the inhibition of microbial growth by antibiotics except

4.
A feature common to almost all transmembrane proteins is

5.
The distribution of transmembrane protein in the palne of a cell membrane can best be visualized by which of the following:

6.
Broker, Chow, Roberts and P. Sharp hybridized mRNA from adenovirus to denatured adenovirus DNA and examined the resultant duplex molecules by electron microscopy. Which of the following did they observe?

7.
If a mutation occurred in the phosphorylation site in the CTD domain of RNA polymerase II, which of the following outcomes would be observed?

8.
A microorganism when viewed under a compound microscope with objective of 40X and eye piece of 10X magnification measured 400μ in length. The same microorganism when viewed under a dissecting microscope with 10X lens, would measure

9.
Which of the following technique is used to inactivate a gene by altering the DNA encoding it?

10.
Gene conversion is observed