Basic Principles of Biotechnology MCQ Questions & Answers

21

Henderson's equation can be respresented as

A) $$pH = p{K_a} + log\frac{{[Acid]}}{{[Salt]}}$$ B) $$pH = p{K_a} + log\frac{{[Salt]}}{{[Acid]}}$$ C) $$p{K_a} = pH + log\frac{{[Salt]}}{{[Acid]}}$$ D) $$p{K_a} = pH - log\frac{{[Salt]}}{{[Acid]}}$$

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Answer & Solution

Answer: Option B

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22

The role of calcium in muscle contraction is to

A) Break the cross-bridges as a cofactor in the hydrolysis of ATP B) Bind with troponin, changing its shape so that the actin filament is exposed C) Transmit the action potential across the neuromuscular junction D) Spread the action potential through the T tubules

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Answer & Solution

Answer: Option B

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23

Why is the inactivation of a useful technique to determine the function of a gene?

A) Gene inactivation provides information about the expression of a gene B) Gene inactivation provides information about the cellular location of a gene product C) Gene inactivation provides an opportunity to identify phenotypic changes associated with the loss of the functional gene D) Gene inactivation provides information about the structure of the gene product

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Answer & Solution

Answer: Option C

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24

Which of the following statements is not true of fatty acids found in most mammalian cells?

A) They usually contain 12 to 20 carbons B) Polyunsaturated fatty acids contain conjugated double bonds C) All double bonds are in the cis configuration D) Free fatty acids are ionized at physiological pH

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Answer & Solution

Answer: Option B

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25

The isoelectric point of a protein is defined as

A) The pH at which the net charge on the molecule is zero B) The pH at which all groups are protonated C) The pH at which all groups are unprotonated D) The pH at which each acidic group is protonated and each basic group is unprotonated

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Answer & Solution

Answer: Option A

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26

Prandtl number is the ratio of

A) Thermal diffusivity to momentum diffusivity B) Mass diffusivity to momentum diffusivity C) Momentum diffusivity to thermal diffusivity D) Thermal diffusivity to mass diffusivity

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Answer & Solution

Answer: Option C

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27

Energy capture efficiency of the aerobic cells using glucose as a substrate is

A) 50% B) 40% C) 30% D) 20%

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Answer & Solution

Answer: Option B

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28

For a nucleic acid probe to hybridize, which of the following must be true?

A) Both the probe and the sequence to which it hybridizes must be RNA B) Both the probe and the sequence to which it hybridizes must be DNA C) The sequence to which it hybridizes must be identical to that of the probe D) The sequence to which it hybridizes must be complementary to that of the probe

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Answer & Solution

Answer: Option D

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29

A homogeneous protein of native molecular weight 100,000 gave a single band of molecular weight 50,000 on SDS-PAGE in presence of β-mercaptoethanol. N-terminal analysis gave two amino acids alanine and leucine in equal proportions. Hence the

A) Protein is a homodimer B) Protein is contaminated with another protein C) Protein has two polypeptides linked by disulphide bridges D) None of the above

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Answer & Solution

Answer: Option A

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30

In the RNAi regulatory pathway, the DICER enzyme cleaves

A) RNA polymerase into non-functional pieces B) Single-stranded DNA into repetitive sequence C) Double-stranded RNA into short strands D) mRNAs of genes to be repressed

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Answer & Solution

Answer: Option C

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Basic Principles of Biotechnology MCQ Questions & Answers | Biotechnology