Bioprocess Engineering and Process Biotechnology MCQ Questions & Answers | Biotechnology

The limiting factor in the production of large quantities of ethanol as biofuel is

If the fractional recovery at each step of unit operation is 0.8, the recovery after 4 steps will be

Diauxic pattern of biomass growth is associated with
P. Multiple lag phases.
Q. Sequential utilization of multiple substrates.
R. Simultaneous utilization of multiple substrates.
S. Absence of lag phase.

Downstream processing of an industrial process yielded a highly purified bioactive protein. This protein was subjected to cleavage by trypsin. Chromatographic separation of products resulted in 4 peptides (P, Q, R, S) with the following amino acid sequences.
P. Phe-Val-Met-Val-Arg
Q. Ala-Ala-Try-Gly-Lys
R. Val-Phe-Met-Ala-Gly-Lys
S. Phe-Gly-Try-Ser-Thr
Chemical cleavage of the same protein with cyanogen bromide and chromatographic separation resulted in three peptides (i, ii, iii) with the following sequences
(i) Ala-Gly-Lys-Phe-Gly-Try-Ser-Thr
(ii) Ala-Ala-Try-Gly-Lys-Phe-Val-Met
(iii) Val-Arg-Val-Phe-Met
The order of the peptides that gives the primary structure of the original protein is

Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are 5.6 and 6.8. Which one of the following techniques can be used to separate them?

Aeration in a bioreactor is provided by

The specific growth rate (μ) of a microorganism in death phase is

Immobilization of enzymes using entrapment method requires
P. Photosensitive polyethylene glycol dimethacrylate.
Q. CNBr activation of sepharose.
R. Polyfunctional reagent like hexamethylene diisocyanate.
S. Radiation of polyvinyl alcohol.

Which of the following techniques is not ideal for immobilizing cell-free enzymes?

Determine the correctness or otherwise of the following Assertion (A) and Reason (R).
Assertion: Bacterial growth is called synchronous when majority of the cells are in same stage of the bacterial cell cycle.
Reason: Synchronous culture can be obtained by growing bacteria in an enriched medium