Answer & Solution
Answer: Option D
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72. If the fractional recovery at each step of unit operation is 0.8, the recovery after 4 steps will be
73. Diauxic pattern of biomass growth is associated with
P. Multiple lag phases.
Q. Sequential utilization of multiple substrates.
R. Simultaneous utilization of multiple substrates.
S. Absence of lag phase.
P. Multiple lag phases.
Q. Sequential utilization of multiple substrates.
R. Simultaneous utilization of multiple substrates.
S. Absence of lag phase.
74. Downstream processing of an industrial process yielded a highly purified bioactive protein. This protein was subjected to cleavage by trypsin. Chromatographic separation of products resulted in 4 peptides (P, Q, R, S) with the following amino acid sequences.
P. Phe-Val-Met-Val-Arg
Q. Ala-Ala-Try-Gly-Lys
R. Val-Phe-Met-Ala-Gly-Lys
S. Phe-Gly-Try-Ser-Thr
Chemical cleavage of the same protein with cyanogen bromide and chromatographic separation resulted in three peptides (i, ii, iii) with the following sequences
(i) Ala-Gly-Lys-Phe-Gly-Try-Ser-Thr
(ii) Ala-Ala-Try-Gly-Lys-Phe-Val-Met
(iii) Val-Arg-Val-Phe-Met
The order of the peptides that gives the primary structure of the original protein is
P. Phe-Val-Met-Val-Arg
Q. Ala-Ala-Try-Gly-Lys
R. Val-Phe-Met-Ala-Gly-Lys
S. Phe-Gly-Try-Ser-Thr
Chemical cleavage of the same protein with cyanogen bromide and chromatographic separation resulted in three peptides (i, ii, iii) with the following sequences
(i) Ala-Gly-Lys-Phe-Gly-Try-Ser-Thr
(ii) Ala-Ala-Try-Gly-Lys-Phe-Val-Met
(iii) Val-Arg-Val-Phe-Met
The order of the peptides that gives the primary structure of the original protein is
75. Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are 5.6 and 6.8. Which one of the following techniques can be used to separate them?
76. Aeration in a bioreactor is provided by
77. The specific growth rate (μ) of a microorganism in death phase is
78. Immobilization of enzymes using entrapment method requires
P. Photosensitive polyethylene glycol dimethacrylate.
Q. CNBr activation of sepharose.
R. Polyfunctional reagent like hexamethylene diisocyanate.
S. Radiation of polyvinyl alcohol.
P. Photosensitive polyethylene glycol dimethacrylate.
Q. CNBr activation of sepharose.
R. Polyfunctional reagent like hexamethylene diisocyanate.
S. Radiation of polyvinyl alcohol.
79. Which of the following techniques is not ideal for immobilizing cell-free enzymes?
80. Determine the correctness or otherwise of the following Assertion (A) and Reason (R).
Assertion: Bacterial growth is called synchronous when majority of the cells are in same stage of the bacterial cell cycle.
Reason: Synchronous culture can be obtained by growing bacteria in an enriched medium
Assertion: Bacterial growth is called synchronous when majority of the cells are in same stage of the bacterial cell cycle.
Reason: Synchronous culture can be obtained by growing bacteria in an enriched medium