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41
Bacillus subtilis under ideal conditions occupies 5% of the total volume of a fermenter. Under such conditions; the culture medium (after bacilli are filtered off) has a concentration of 1mg/ml of the secreted protein trehalae
(i) Calculate the volume of culture filtrate required to purify 1 kg of trehalase
(ii) What are the total number of B. subtilis cells which produce this amount of enzyme? Assume that each cell is a cylinder which has dimensions of 1 m diameter and length 2μm.
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Answer: Option D
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42
In animal cell cultures, the addition of serum to media is essential for providing
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Answer: Option D
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43
A knockout mouse is so named because it contains
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Answer: Option C
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44
In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is correct?
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Answer: Option A
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45
Which of these would not Be-used to introduce DNA in animal cells?
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Answer: Option D
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46
The first patent for a transgenic organism was awarded in 1988. Which organism was patented?
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Answer: Option C
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47
Carbon-dioxide when combined with the amino groups in haemoglobin molecule and forms
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Answer: Option A
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48
Term hybridoma implies
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Answer: Option C
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49
DNA ligase of E. coli requires which of the following cofactors?
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Answer: Option D
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50
The syndrom es in human in which an individual's somatic cells contain only the one sex chromosome XO is called
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Answer: Option B
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