Animal Biotechnology MCQ Questions & Answers

51

Choose the correct sequence of steps involved in cytoplast production.

A) Digestion of cell wall → Protoplast viability → Cybrid formation → Osmotic stabilizer B) Osmotic stabilizer → Digestion of cell wall → Protoplast viability → Cybrid formation C) Protoplast viability → Osmotic stabilizer → Digestion of cell wall → Cybrid formation D) Osmotic stabilizer → Digestion of cell wall → Cybrid formation → Protoplast viability

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Answer & Solution

Answer: Option B

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52

Which one of the following statements is incorrect about hybridoma production?

A) Hybridoma cells can use hypoxanthine and thymidine B) DNA synthesis in myeloma cells is blocked by aminopterin C) Hybridoma cells are made to produce polyclonal antibodies D) Polyethylene glycol is used to fuse myeloma cells to B-cells

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Answer & Solution

Answer: Option C

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53

Antibody-producing hybridoma cells are generated by the fusion of a

A) T cell with a myeloma cell B) B cell with a myeloma cell C) Macrophage with a myeloma cell D) T cell and a B cell

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Answer & Solution

Answer: Option B

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54

Which of the following has been produced commercially from mammalian cell cultures?

A) Plasminogen activator B) Antibacterial antibiotics C) Insulin D) Renin

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Answer & Solution

Answer: Option A

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55

When the value of selection coefficient is unity (S = 1), there would be

A) No survival B) 10% survival C) 50% survival D) 75% survival

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Answer & Solution

Answer: Option A

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56

To promote attachment and spreading of anchorage-dependent animal cells the surface of the culture vessel needs to be coated with

A) Trypsin B) Collagen C) Pronase D) Polyglycol

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Answer & Solution

Answer: Option B

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57

The culture fluids of 1000 to 5000 colonies of hybridoma are screened for monoclonal antibody by
P. Western blot analysis.
Q. Antigen capture analysis.
R. Northern blot analysis.
S. Antibody capture analysis.
Choose the correct pair from the following

A) P, Q B) Q, R C) R, S D) Q, S

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Answer & Solution

Answer: Option A

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58

Nitrogen fixing genes are called

A) Nif' genes B) Plasmid genes C) Leg genes D) Cos genes

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Answer & Solution

Answer: Option A

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59

Transgenic pharming involves using . . . . . . . . to produce recombinant proteins in . . . . . . . .

A) Cows; Milk B) Phage; Bacteria C) Bacteria; Culture media D) Fungi; Culture media

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Answer & Solution

Answer: Option A

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60

The genes which promote cancerous growth are called as

A) Oncogenes B) Tumour suppressor genes C) Both A and B D) None of the above

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Answer & Solution

Answer: Option A

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Animal Biotechnology MCQ Questions & Answers | Biotechnology